Port a patch nanion

An optional Perfusion System and Temperature Control device is available. With conventional patch clamp this is difficult because of the tiny dimensions of the pipette tip, the long tapered shaft and, thus, the diffusion limitation of the liquid exchange. This allows for novel and complex experiments on interactions between ion channels and drugs.

Another aspect of internal perfusion is the possibility to add membrane permeable drugs directly to the inside of the cell and, thereby, accelerating the time course of the experiment. Manual internal solution exchange Using the Port-a-Patch, the internal solution can be exchanged using a pipette. As an example, pore forming compounds like Nystatin can be used to perforate the membrane patch in order to obtain electrical control over the membrane.

Perforated and conventional whole cell configuration and derived Kv1. In our recordings performed with the Port-a-Patch we could confirm this observation. The voltage-dependence was shifted significantly to more negative potentials in the whole cell configuration compared to the perforated whole cell configuration.

Whole cell currents after a conventional membrane breakthrough left and perforated whole cell currents middle. Nystatin was added to the internal solution after the cell attached configuration was reached. I-V curves of peak current of 3 conventional and 5 perforated cells right. It allows accurate application of up to eight different compounds and solutions. The micro-controller based design makes it compatible with all commonly used patch clamp software solutions.

Together with the dedicated perfusion chamber for the Port-a-Patch, it is a simple system for automated perfusion experiments. The system is fully software controlled, but can also be used in a manual mode. Furthermore, the system can be upgraded for temperature control.

Exchange times for all solution exchanges were approx. The Port-a-Patch SOL enables optical stimulation during recordings on the Port-a-Patch by covering it with a dedicated lid to which up to three light sources can be connected. The light source can either controlled manually or by the amplifier software. The Add-On does not include a light source, so you are free to use light sources you already have.

PatchControl Software. PatchControl is a software programme written with the user in mind. Pre-written protocols can be loaded and altered, allowing the entire procedure for cell capture, sealing and obtaining the whole cell configuration to be completely automated. However, because we know that sometimes you just want to control things yourself, the protocols can be interrupted at any time and the suction controlled manually.

Through innovative communication with electrophysiology software, PatchControl can read important parameters such as membrane resistance, whole cell capacitance and series resistance and moves through the pre-programmed steps accordingly.

Parameters such as suction strength and duration, voltage and membrane criteria can be set and altered to obtain the optimum parameters for cells routinely used in your lab. Not only that, complex macros can be easily implemented, allowing everything from simple changes in gain to complex perfusion routines to be programmed and automatically performed.

The PatchControl software is there to make your patch clamp life easier, from automated cell capture to automated pressure control, the PatchControl software can do it all! It is produced and quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers. Different types of NPC-1 chips are available which should be chosen depending on cell size and application. Reliable buffer solutions are critical for any electrophysiological application.

Our goal, therefore, is to provide high-class recording solutions that leave you in no doubt about quality and stability.

Our quality assurance includes chemical tests as well as tests on our patch clamp systems of each lot. We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience tracking cookies. You can decide for yourself whether you want to allow cookies or not.

Please note that if you reject them, you may not be able to use all the functionalities of the site. How it Works Application Areas. Dynamic Clamp: Patchliner. Bilayer recordings: Orbit product family. Port-a-Patch and Patchliner Webinar. Port-a-Patch Webinar. The Webinar focuses on the automated patch clamp assay development for the study of red blood cells in health and disease and the RELEVANCE project , an international consortium of 13 partners from academia, diagnostic labs, blood supply centers, and small companies that combines basic and translational research to improve prognostic, diagnostic and therapeutic approaches on red blood cell function in health and disease.

To this end, Nanion contributes assays for the electrophysiological characterization of healthy and patient-derived red blood cells. This webinar shows applications that go way beyond possibilities of conventional patch-clamping, where the Port-a-Patch facilitates completely novel scientific directions. Coli spheroplasts" Port-a-Patch application note: 0.

Port-a-Patch application note: 0. Port-a-Patch and Vesicle Prep Pro application note: 0. Port-a-Patch application note 0. Port-a-Patch and Vesicle Prep Pro application note 0. Port-a-Patch and Vesicle Prep Pro application note: 1.

Port-a-Patch application note: 1. Port-a-Patch application note: 3. Port-a-Patch product sheet: 1. Port-a-Patch product flyer 0. Port-a-Patch Publication in Frontiers in Pharmacology Authors: Kambayashi R.

Port-a-Patch Publication in Haematologica Authors: Belkacemi A. Authors: Bin Nadzirin I. Port-a-Patch Publication in Toxins Authors: Liu Y. Authors: Najbauer E. Port-a-Patch Publication in Molecular Pharmacology Authors: Bidula S. Authors: Xu Y.

Authors: Proks P. Port-a-Patch Publication in Lab Invest Authors: Turan E. Port-a-Patch prepublication in bioRxiv Authors: Bernhard M. Authors: Bartsch A. Port-a-Patch Publication in Front. Authors: Lukacs P. Authors: Li G. Authors: Costa B. Authors: Peng K. Authors: Sancho-Vaello E. Authors: Molbaek K. Port-a-Patch publication in Nature Scientific Reports Port-a-Patch pre-publication in BioRxiv Authors: Debnath M. Port-a-Patch publication in Cellular and Molecular Neurobiology Authors: Iwahasi Y.

Port-a-Patch publication in Clinical Science Authors: Chen J. Port a Patch publication in Laboratory Investigation Authors: Oronowicz J. Authors: Bhat O. Authors: Lagu B. Authors: Manzo G. Authors: Wang K. Port-a Patch Publication in Scientific Reports Authors: Bodi I. Port-a Patch Publication in Cancers Authors: Liu X. Authors: Poveda J. Authors: Tanner M. Port-a-Patch publication in Cancers Authors: Sierla M.

Port-a-Patch publication in Neuroscience Letters Port-a-Patch publication in British Journal of Pharmacology Authors: Hammelmann, V. Port-a-Patch publication in Frontiers in Pharmacology Authors: Walcher, L.

Authors: Hu L. Authors: Zhang M. Authors: Wu G. Authors: Klaerke D. Port-a-Patch publication in Science Advances Authors: Agboh K. Authors: Liu, X. Port-a-Patch and Orbit mini article in Protein Engineering Port-a-Patch publication in Circulation Authors: Yao C. Port-a-Patch publication in Microgravity Port-a-Patch publication in Journal of General Physiology Port-a-Patch publication in Frontiers in Endocrinology Port-a-Patch publication in Angewandte Chemie Port-a-Patch publication in Clinical Immunology Authors: Rangel M.

Authors: Kazuma K. Authors: Shapovalov G. Port-a-Patch publication in Oncotarget Authors: Silva J. Port-a-Patch publication in Cell Proliferation Authors: Calvez M. Authors: Arya P, Jarerattanachat V. Authors: Clausen M. Port-a-Patch publication in Journal of Biological Chemistry Port-a-Patch publication in Biophysical Journal Authors: Briones R.

Authors: Garreis F. Authors: Survery S. Port-a-Patch publication in Scientific Reports Authors: Urban P. Authors: Babes A. Authors: Moparthi L. Authors: Soranzo T. Authors: Lidgerwood G. Patchliner publication in Journal of the American College of Cardiology Authors: Lorberbaum T.

Port-a-Patch publication in Biochemistry Authors: Meng x. Port-a-Patch publication in Biotechnology Reports Authors: Kikuchi K. Authors: Cama J. Port-a-Patch publication in Science Authors: Dong Y. Authors: Carrara G. Authors: Billen B. Authors: Weichbrodt C. Authors: Stava E. Authors: Kniggendorf A. Authors: Barthmes M. Authors: Dondapati S. Port-a-Patch publication in Cellular Physiology and Biochemistry